![](https://en.hcmus.edu.vn/wp-content/uploads/2023/09/20201203-Hinh-the-Hoang-Nguyen-Duc-Hoang-e1694746141851.jpg)
Center for Science and Biotechnology
Position | Director of the Center for Science and Biotechnology |
Citation name | Hoang Nguyen, Hoang Duc Nguyen, Hoang D Nguyen https://bit.ly/NguyenDucHoang-profile |
Field of Professional | Bacterial genetics, Biotechnology |
Year of appointment title of Associate Professor | 2015 |
Style of citation | N/A |
Academic background | In 1998 VNUHCM-University of Science BSc of Biology In 2002 VNUHCM-University of Science MSc of Microbiology In 2006 University of Bayreuth, Germany DSc of Natural Sciences |
Name Languages | ASSOC.PROF. NGUYỄN ĐỨC HOÀNG ndhoang@hcmus.edu.vn Vietnamese, English |
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PUBLICATION
- Nguyen HD, Nguyen QA, Ferreira RC, Ferreira LC, Tran LT, Schumann W. 2005. Construction of plasmid-based expression vectors for Bacillus subtilis exhibiting full structural stability. Plasmid 54: 241-248.
- Nguyen HD, Schumann W. 2006. Establishment of an experimental system allowing immobilization of proteins on the surface of Bacillus subtilis cells. J Biotechnol 122: 473-482.
- Phan TTP, Nguyen HD, Schumann W. 2006. Novel plasmid-based expression vectors for intra- and extracellular production of recombinant proteins in Bacillus subtilis. Protein Expr Purif 46: 189-195.
- Shibasaki S, Kuroda K, Nguyen HD, Mori T, Zou W, Ueda M. 2006. Detection of protein-protein interactions by a combination of a novel cytoplasmic membrane targeting system of recombinant proteins and fluorescence resonance energy transfer. Appl Microbiol Biotechnol 70: 451-457.
- Paccez JD, Nguyen HD, Luiz WB, Ferreira RC, Sbrogio-Almeida ME, Schumann W, Ferreira LC. 2007. Evaluation of different promoter sequences and antigen sorting signals on the immunogenicity of Bacillus subtilis vaccine vehicles. Vaccine 25: 4671-4680.
- Nguyen HD, Phan TTP, Schumann W. 2007. Expression vectors for the rapid purification of recombinant proteins in Bacillus subtilis. Curr Microbiol 55: 89-93.
Uhlenheuer DA, Nguyen HD, Wasserberg D, Zhang L, Blum C, Subramaniam V, Brunsveld L. 2009. Modulation of protein dimerization by a supramolecular host-guest system. Chemistry 15: 8779-8790. - Young JF, Nguyen HD, Yang L, Huskens J, Jonkheijm P, Brunsveld L. 2010. Strong and reversible monovalent supramolecular protein immobilization. Chembiochem 11: 180-183.
- Nguyen HD, Dang DT, van Dongen JL, Brunsveld L. 2010. Protein Dimerization Induced by Supramolecular Interactions with Cucurbit[8]uril. Angew Chem Int Ed Engl 49: 895-898.
- Phan T, Nguyen HD, Goksel H, Mocklinghoff S, Brunsveld L. 2010. Phage display selection of miniprotein binders of the Estrogen Receptor. Chem Commun (Camb ) 46: 8207-8209.
- Phan TT, Nguyen HD, Schumann W. 2010. Establishment of a simple and rapid method to screen for strong promoters in Bacillus subtilis. Protein Expr Purif 71: 174-178.
- Uhlenheuer DA, Young JF, Nguyen HD, Scheepstra M, Brunsveld L. 2011. Cucurbit[8]uril induced heterodimerization of methylviologen and naphthalene functionalized proteins. Chem Commun (Camb ) 47: 6798-6800.
- Nguyen HD, Phan TT, Schumann W. 2011. Analysis and application of Bacillus subtilis sortases to anchor recombinant proteins on the cell wall. AMB Express 1: 22.
- Getlik M, Grutter C, Simard JR, Nguyen HD, Robubi A, Aust B, van Otterlo WA, Rauh D. 2012. Structure-based design, synthesis and biological evaluation of N-pyrazole, N’-thiazole urea inhibitors of MAP kinase p38α. Eur J Med Chem 48: 1-15.
- Phan TT, Nguyen HD, Schumann W. 2012. Development of a strong intracellular expression system for Bacillus subtilis by optimizing promoter elements. J Biotechnol 157: 167-172.
- Uhlenheuer DA, Wasserberg D, Haase C, Nguyen HD, Schenkel JH, Huskens J, Ravoo BJ, Jonkheijm P, Brunsveld L. 2012. Directed Supramolecular Surface Assembly of SNAP-tag Fusion Proteins. Chemistry 18: 6788-6794.
- Nguyen HD, Phan TT, Carraz M, Brunsveld L. 2012. Estrogen Receptor alpha/beta-cofactor motif interactions; interplay of tyrosine 537/488 phosphorylation and LXXLL motifs. Mol Biosyst 8: 3134-3141
- Yang L, Gomez-Casado A, Young JF, Nguyen HD, Danés JC, Huskens J, Brunsveld L, Jonkheijm P. 2012. Reversible and Oriented Immobilization of Ferrocene-modified Proteins. JACS 134: 19199-19206
- Dang DT, Nguyen HD, Merkx M, Brunsveld L. 2013. Supramolecular Control of Enzyme Activity through Cucurbit[8]uril-Mediated Dimerization. Angew Chem Int Ed Engl. 52(10): 2915–2919.
- Fuchs S*, Nguyen HD*, Phan TTP, Burton MF, Nieto L, Leeuwen IJV, Schmidt A, Goodarzifard M, Agten SM, Rose R, Ottmann C, Milroy LG, Brunsveld L. 2013. Proline Primed Helix Length as a Modulator of the Nuclear Receptor-Coactivator Interaction. JACS. 135: 4364-4371. (*, first author shared)
- Phan TTP, Nguyen HD, Wolfgang Schumann. 2013. Construction of a 5′-controllable stabilizing element (CoSE) for over-production of heterologous proteins at high levels in Bacillus subtilis. J Biotechnol. 168(1): 32-39.
- Richters A, Nguyen H, Phan T, Simard J, Grütter C, Engel J, Rauh D. 2014. Identification of Type II and Type III DDR2 Inhibitors. J. Med. Chem. 57(10):4252-62.
- Fang Z, Simard JR, Plenker D, Nguyen HD, Phan T, Wolle P, Baumeister S, Rauh D. 2015. Discovery of Inter-Domain Stabilizers – A Novel Assay System for Allosteric Akt Inhibitors. ACS Chemical Biology 10(1): 279-288
- Phan TTP, Tran LT, Schumann W, Nguyen HD.* 2015. Development of Pgrac100-based expression vectors allowing high protein production levels in Bacillus subtilis and relatively low basal expression in Escherichia coli, Microbial Cell Factories 14:72
- Tran TA, Vo TN, Nguyen HD, Pham TB. 2015. A novel method to predict Highly Expressed Genes based on radius clustering and relative synonymous codon usage, Journal of computational biology 22(12):1986-1096.
- Becker C, Öcal S, Nguyen HD, Phan T, Keul M, Simard JR, Rauh D. 2016. Monitoring conformational changes in the receptor tyrosine kinase EGFR, ChemBioChem 17(11): 990-994.
- Tran DTM, Phan TTP, Huynh TK, Dang NTK, Huynh PTK, Nguyen TM, Truong TTT, Tran TL, Schumann W, Nguyen HD.* 2017. Development of inducer-free expression plasmids based on IPTG-inducible promoters for Bacillus subtilis, Microbial Cell Factories 16: 130.
- Le V, Phan T, Nguyen T, Brunsveld L, Schumann W, Nguyen H.* 2019. Using the IPTG-Inducible Pgrac212 Promoter for Overexpression of Human Rhinovirus 3C Protease Fusions in the Cytoplasm of Bacillus subtilis Cells, Current Microbiology 76(12) 1477-1486 DOI: 10.1007/s00284-019-01783-9
- Tran DM, Phan TTP, Doan TTN, Tran TL, Schumann W, Nguyen HD*. 2020. Integrative expression vectors with Pgrac promoters for inducer-free overproduction of recombinant proteins in Bacillus subtilis, Biotechnology Reports 28 DOI: 10.1016/j.btre.2020.e00540
- Dung Thanh Dang, Le Tuan Anh Nguyen, Tuom Tinh Thi Truong, Hoang Duc Nguyen and Anh Tuân Phan. 2021. Construction of a G-quadruplex-specific DNA endonuclease Chem. Commun. 57(37), 4568-4571
- T. Truong, H. D. Bui, T. T. V. Pham, L. T. Tran, D. H. Nguyen, C. Ng & T.-H. Le. 2021. Occurrences of antibiotic resistant bacteria in a tropical river impacted by anthropogenic activities in Ho Chi Minh City, International Journal of Environmental Science and Technology.
- Hefele L, Black AP, Trinh VT, Nguyen TM, Nguyen DH, Virachith S, Muller CP, Hübschen JM, Russell P, Bartholdson SJ, Nguyen NMC, Tran VTN, Baker S. 2021. An age-stratified serosurvey against purified Salmonella enterica serovar Typhi antigens in the Lao People´s Democratic Republic, PLoS Negl Trop Dis 15(12): e0010017
- Nhi NY Nguyen, Lan NH Duong, Dat Duc Truong, Tam Th Nguyen, An K Nguyen, Thanh Tien Nguyen, Uyen TT Nguyen, Thang Mai Dinh, Dong Le Thanh, Hoang Duc Nguyen*. 2021. Generation of Bacillus subtilis displaying alpha-toxin HlaH35LH48L fused with CotB and CotG, and studying the immune response in mice, Biomedical research and therapy 8(12): 4793-4802.
- Hoang D. Nguyen*Trang T. P. Phan. 2022. A Protocol to Enhance Soluble Protein Expression in the Cytoplasm of Bacillus subtilis, Methods in Molecular Biology: Insoluble Protein 2406: 233-243.
- Ngan T.P. Le, Trang T.P. Phan, Hanh T.T. Phan, Tuom T.T. Truong, Wolfgang Schumann, Hoang D. Nguyen*. 2022. Influence of N-terminal His-tags on the production of recombinant proteins in the cytoplasm of Bacillus subtilis, Biotechnology Reports 35: e00754.
- T. H. Le, T. Truong, L.T. Tran, D.H. Nguyen, T. P. T. Pham, C. Ng. 2022. Antibiotic resistance in the aquatic environments: the need for an interdisciplinary approach, International Journal of Environmental Science and Technology,
- Tuom Thi Tinh Truong, Viet Quoc Huynh, Nam Tri Vo, and Hoang Duc Nguyen*. 2022. Studying the characteristics of nanobody CDR regions based on sequence analysis in combination with 3D structures, Journal of genetic engineering and biotechnology 20: 157
- Thuy Trang Dinh, Trang Phuong Vo-Chanh, Chau Nguyen, Viet Quoc Huynh, Nam Vo & Hoang Duc Nguyen*, 2022. Extract antibody and antigen names from biomedical literature, BMC Bioinformatics, 23: 524
As the leader of the projects
- Phát triển vaccine ngâm và cho ăn phòng bệnh do Aeromonas hydrophila và Edwardsiella ictulari ở cá tra sử dụng vector chuyển vaccine Bacillus subtilis (VINIF.2021.DA00034 (VinIF, Vingroup)
- Tạo các quyết định độc tố LukF/S-PV, các biến thể của alpha-toxin và ClfA ở Bacillus subtilis và phát triển vector phân phối vaccine B. subtilis kháng lại Staphylococcus aureus (Nafosted, 108.06-2019.11)
- Chọn lọc trình tự DNA mã hóa đuôi His-tag và kết hợp với các đuôi dung hợp khác ở đầu N nhằm tăng cường biểu hiện protein tái tổ hơp trong Bacillus subtilis (B2019-18-10; Đại học Quốc gia)
- Tạo dòng, biểu hiện và thu nhận Tobaco Etch Virus Protease (TEV) và Human Rhinovirus 3C Protease (HRV3C) ở Bacillus subtilis [Sở Khoa học và Công nghệ TP. HCM (Số 180/HĐ-SKHCN)]
- Development of IPTG-inducible integration expression vectors and auto-inducible expression vectors based on Pgrac promoter for Bacillus subtilis [14-201 RG/BIO/AS_G,TWAS (the Academy of Sciences for the developing world, UNESCO FR: 324028608)]
- Development of dual plasmid-based expression vectors for both Bacillus subtilis and Escherichia coli (11-188 RG/BIO/AS_G,TWAS [the Academy of Sciences for the developing world, UNESCO FR: 3240262709)]