Center for Science and Biotechnology

PositionDirector of the Center for Science and Biotechnology
Citation nameHoang Nguyen, Hoang Duc Nguyen, Hoang D Nguyen
Field of ProfessionalBacterial genetics, Biotechnology
Year of appointment title of Associate Professor2015
Style of citationN/A
EducationIn 1998
VNUHCM-University of Science
BSc of Biology
In 2002
VNUHCM-University of Science
MSc of Microbiology
In 2006
University of Bayreuth, Germany
DSc of Natural Sciences
Vietnamese, English


  1. Nguyen HD, Nguyen QA, Ferreira RC, Ferreira LC, Tran LT, Schumann W. 2005. Construction of plasmid-based expression vectors for Bacillus subtilis exhibiting full structural stability. Plasmid 54: 241-248.
  2. Nguyen HD, Schumann W. 2006. Establishment of an experimental system allowing immobilization of proteins on the surface of Bacillus subtilis cells. J Biotechnol 122: 473-482.
  3. Phan TTP, Nguyen HD, Schumann W. 2006. Novel plasmid-based expression vectors for intra- and extracellular production of recombinant proteins in Bacillus subtilis. Protein Expr Purif 46: 189-195.
  4. Shibasaki S, Kuroda K, Nguyen HD, Mori T, Zou W, Ueda M. 2006. Detection of protein-protein interactions by a combination of a novel cytoplasmic membrane targeting system of recombinant proteins and fluorescence resonance energy transfer. Appl Microbiol Biotechnol 70: 451-457.
  5. Paccez JD, Nguyen HD, Luiz WB, Ferreira RC, Sbrogio-Almeida ME, Schumann W, Ferreira LC. 2007. Evaluation of different promoter sequences and antigen sorting signals on the immunogenicity of Bacillus subtilis vaccine vehicles. Vaccine 25: 4671-4680.
  6. Nguyen HD, Phan TTP, Schumann W. 2007. Expression vectors for the rapid purification of recombinant proteins in Bacillus subtilis. Curr Microbiol 55: 89-93.
    Uhlenheuer DA, Nguyen HD, Wasserberg D, Zhang L, Blum C, Subramaniam V, Brunsveld L. 2009. Modulation of protein dimerization by a supramolecular host-guest system. Chemistry 15: 8779-8790.
  7. Young JF, Nguyen HD, Yang L, Huskens J, Jonkheijm P, Brunsveld L. 2010. Strong and reversible monovalent supramolecular protein immobilization. Chembiochem 11: 180-183.
  8. Nguyen HD, Dang DT, van Dongen JL, Brunsveld L. 2010. Protein Dimerization Induced by Supramolecular Interactions with Cucurbit[8]uril. Angew Chem Int Ed Engl 49: 895-898.
  9. Phan T, Nguyen HD, Goksel H, Mocklinghoff S, Brunsveld L. 2010. Phage display selection of miniprotein binders of the Estrogen Receptor. Chem Commun (Camb ) 46: 8207-8209.
  10. Phan TT, Nguyen HD, Schumann W. 2010. Establishment of a simple and rapid method to screen for strong promoters in Bacillus subtilis. Protein Expr Purif 71: 174-178.
  11. Uhlenheuer DA, Young JF, Nguyen HD, Scheepstra M, Brunsveld L. 2011. Cucurbit[8]uril induced heterodimerization of methylviologen and naphthalene functionalized proteins. Chem Commun (Camb ) 47: 6798-6800.
  12. Nguyen HD, Phan TT, Schumann W. 2011. Analysis and application of Bacillus subtilis sortases to anchor recombinant proteins on the cell wall. AMB Express 1: 22.
  13. Getlik M, Grutter C, Simard JR, Nguyen HD, Robubi A, Aust B, van Otterlo WA, Rauh D. 2012. Structure-based design, synthesis and biological evaluation of N-pyrazole, N’-thiazole urea inhibitors of MAP kinase p38α. Eur J Med Chem 48: 1-15.
  14. Phan TT, Nguyen HD, Schumann W. 2012. Development of a strong intracellular expression system for Bacillus subtilis by optimizing promoter elements. J Biotechnol 157: 167-172.
  15. Uhlenheuer DA, Wasserberg D, Haase C, Nguyen HD, Schenkel JH, Huskens J, Ravoo BJ, Jonkheijm P, Brunsveld L. 2012. Directed Supramolecular Surface Assembly of SNAP-tag Fusion Proteins. Chemistry 18: 6788-6794.
  16. Nguyen HD, Phan TT, Carraz M, Brunsveld L. 2012. Estrogen Receptor alpha/beta-cofactor motif interactions; interplay of tyrosine 537/488 phosphorylation and LXXLL motifs. Mol Biosyst 8: 3134-3141
  17. Yang L, Gomez-Casado A, Young JF, Nguyen HD, Danés JC, Huskens J, Brunsveld L, Jonkheijm P. 2012. Reversible and Oriented Immobilization of Ferrocene-modified Proteins. JACS 134: 19199-19206
  18. Dang DT, Nguyen HD, Merkx M, Brunsveld L. 2013. Supramolecular Control of Enzyme Activity through Cucurbit[8]uril-Mediated Dimerization. Angew Chem Int Ed Engl. 52(10): 2915–2919.
  19. Fuchs S*, Nguyen HD*, Phan TTP, Burton MF, Nieto L, Leeuwen IJV, Schmidt A, Goodarzifard M, Agten SM, Rose R, Ottmann C, Milroy LG, Brunsveld L. 2013. Proline Primed Helix Length as a Modulator of the Nuclear Receptor-Coactivator Interaction. JACS. 135: 4364-4371. (*, first author shared)
  20. Phan TTP, Nguyen HD, Wolfgang Schumann. 2013. Construction of a 5′-controllable stabilizing element (CoSE) for over-production of heterologous proteins at high levels in Bacillus subtilis. J Biotechnol. 168(1): 32-39.
  21. Richters A, Nguyen H, Phan T, Simard J, Grütter C, Engel J, Rauh D. 2014. Identification of Type II and Type III DDR2 Inhibitors. J. Med. Chem. 57(10):4252-62.
  22. Fang Z, Simard JR, Plenker D, Nguyen HD, Phan T, Wolle P, Baumeister S, Rauh D. 2015. Discovery of Inter-Domain Stabilizers – A Novel Assay System for Allosteric Akt Inhibitors. ACS Chemical Biology 10(1): 279-288
  23. Phan TTP, Tran LT, Schumann W, Nguyen HD.* 2015. Development of Pgrac100-based expression vectors allowing high protein production levels in Bacillus subtilis and relatively low basal expression in Escherichia coli, Microbial Cell Factories 14:72
  24. Tran TA, Vo TN, Nguyen HD, Pham TB. 2015. A novel method to predict Highly Expressed Genes based on radius clustering and relative synonymous codon usage, Journal of computational biology 22(12):1986-1096.
  25. Becker C, Öcal S, Nguyen HD, Phan T, Keul M, Simard JR, Rauh D. 2016. Monitoring conformational changes in the receptor tyrosine kinase EGFR, ChemBioChem 17(11): 990-994.
  26. Tran DTM, Phan TTP, Huynh TK, Dang NTK, Huynh PTK, Nguyen TM, Truong TTT, Tran TL, Schumann W, Nguyen HD.* 2017. Development of inducer-free expression plasmids based on IPTG-inducible promoters for Bacillus subtilis, Microbial Cell Factories 16: 130.
  27. Le V, Phan T, Nguyen T, Brunsveld L, Schumann W, Nguyen H.* 2019. Using the IPTG-Inducible Pgrac212 Promoter for Overexpression of Human Rhinovirus 3C Protease Fusions in the Cytoplasm of Bacillus subtilis Cells, Current Microbiology 76(12) 1477-1486 DOI: 10.1007/s00284-019-01783-9
  28. Tran DM, Phan TTP, Doan TTN, Tran TL, Schumann W, Nguyen HD*. 2020. Integrative expression vectors with Pgrac promoters for inducer-free overproduction of recombinant proteins in Bacillus subtilis, Biotechnology Reports 28 DOI: 10.1016/j.btre.2020.e00540
  29. Dung Thanh Dang, Le Tuan Anh Nguyen, Tuom Tinh Thi Truong,   Hoang Duc Nguyen and  Anh Tuân Phan. 2021. Construction of a G-quadruplex-specific DNA endonuclease Chem. Commun. 57(37), 4568-4571
  30. T. Truong, H. D. Bui, T. T. V. Pham, L. T. Tran, D. H. Nguyen, C. Ng & T.-H. Le. 2021. Occurrences of antibiotic resistant bacteria in a tropical river impacted by anthropogenic activities in Ho Chi Minh City, International Journal of Environmental Science and Technology.
  31. Hefele L, Black AP, Trinh VT, Nguyen TM, Nguyen DH, Virachith S, Muller CP, Hübschen JM, Russell P, Bartholdson SJ, Nguyen NMC, Tran VTN, Baker S. 2021. An age-stratified serosurvey against purified Salmonella enterica serovar Typhi antigens in the Lao People´s Democratic Republic, PLoS Negl Trop Dis 15(12): e0010017
  32. Nhi NY Nguyen, Lan NH Duong, Dat Duc Truong, Tam Th Nguyen, An K Nguyen, Thanh Tien Nguyen, Uyen TT Nguyen, Thang Mai Dinh, Dong Le Thanh, Hoang Duc Nguyen*. 2021. Generation of Bacillus subtilis displaying alpha-toxin HlaH35LH48L fused with CotB and CotG, and studying the immune response in mice, Biomedical research and therapy 8(12): 4793-4802.
  33. Hoang D. Nguyen*Trang T. P. Phan. 2022. A Protocol to Enhance Soluble Protein Expression in the Cytoplasm of Bacillus subtilis, Methods in Molecular Biology: Insoluble Protein 2406: 233-243.
  34. Ngan T.P. Le, Trang T.P. Phan, Hanh T.T. Phan, Tuom T.T. Truong, Wolfgang Schumann, Hoang D. Nguyen*. 2022. Influence of N-terminal His-tags on the production of recombinant proteins in the cytoplasm of Bacillus subtilis, Biotechnology Reports 35: e00754.
  35. T. H. Le, T. Truong, L.T. Tran, D.H. Nguyen, T. P. T. Pham, C. Ng. 2022. Antibiotic resistance in the aquatic environments: the need for an interdisciplinary approach, International Journal of Environmental Science and Technology,
  36. Tuom Thi Tinh Truong, Viet Quoc Huynh, Nam Tri Vo, and Hoang Duc Nguyen*. 2022. Studying the characteristics of nanobody CDR regions based on sequence analysis in combination with 3D structures, Journal of genetic engineering and biotechnology 20: 157
  37. Thuy Trang Dinh, Trang Phuong Vo-Chanh, Chau Nguyen, Viet Quoc Huynh, Nam Vo & Hoang Duc Nguyen*, 2022. Extract antibody and antigen names from biomedical literature, BMC Bioinformatics, 23: 524

As the leader of the projects
  1. Phát triển vaccine ngâm và cho ăn phòng bệnh do Aeromonas hydrophila và Edwardsiella ictulari ở cá tra sử dụng vector chuyển vaccine Bacillus subtilis (VINIF.2021.DA00034 (VinIF, Vingroup)
  2. Tạo các quyết định độc tố LukF/S-PV, các biến thể của alpha-toxin và ClfA ở Bacillus subtilis và phát triển vector phân phối vaccine B. subtilis kháng lại Staphylococcus aureus (Nafosted, 108.06-2019.11)
  3. Chọn lọc trình tự DNA mã hóa đuôi His-tag và kết hợp với các đuôi dung hợp khác ở đầu N nhằm tăng cường biểu hiện protein tái tổ hơp trong Bacillus subtilis (B2019-18-10; Đại học Quốc gia)
  4. Tạo dòng, biểu hiện và thu nhận Tobaco Etch Virus Protease (TEV) và Human Rhinovirus 3C Protease (HRV3C) ở Bacillus subtilis [Sở Khoa học và Công nghệ TP. HCM (Số 180/HĐ-SKHCN)]
  5. Development of IPTG-inducible integration expression vectors and auto-inducible expression vectors based on Pgrac promoter for Bacillus subtilis [14-201 RG/BIO/AS_G,TWAS (the Academy of Sciences for the developing world, UNESCO FR: 324028608)]
  6. Development of dual plasmid-based expression vectors for both Bacillus subtilis and Escherichia coli (11-188 RG/BIO/AS_G,TWAS [the Academy of Sciences for the developing world, UNESCO FR: 3240262709)]